TABLE 1.
Bacterial strains and plasmids used
| Strain and plasmid | Description | References |
|---|---|---|
| Strains | ||
| F. nucleatum 23726 | Parental strain (wild-type strain) | 56 |
| F. nucleatum 51191 | Wild-type strain | From ATCC |
| F. nucleatum CW1 | ∆galK: an isogenic derivative of 23726 | 28 |
| F. nucleatum CW2 | ∆ftsX: an isogenic derivative of CW1 | 28 |
| F. nucleatum CW2c1 | CW2-containing pCWU6a-xylR-E-ftsX | This study |
| F. nucleatum BG01 | WT-containing pBCG06 | This study |
| F. nucleatum ZP01 | ∆tnaA: an isogenic derivative of 23726 | 32 |
| F. nucleatum ZP01c | ZP01-containing pBCG07 | This study |
| F. nucleatum BG02 | lepB conditional mutant strain | This study |
| E. coli DH5α | Cloning host | |
| E. coli C2987 | Cloning host | |
| Plasmids | ||
| pCWU6 | E. coli/Fusobacterium shuttle vector, chloramphenicol/thiamphenicol resistance; cmR/thiaR | 28 |
| pXCH | A xylose-inducible expression vector in an inducible expression vector in Clostridium perfringens | 35 |
| pG106 pCM-galK | A shuttle vector for molecular cloning in Porphyromonas, Bacteroides, and related bacteria Clostridium perfringens vector expressing galK | 57 |
| pgalK--∆lepB | Derivative of pCM-galK, deletion vector of lepB | This study |
| pBCG10 | Derivative of pCM-galK, the ermF-ermAM cassette replaces catP gene | This study |
| pBCG05 | Derivative of pCWU6, KanR | This study |
| pBCG06 | pBCG05 expressing luciferase gene (Luciola red) under the control of xylose-inducible system combination with riboswitch E (pBCG05-xylR-E-luciferase) | This study |
| pBCG06a | pBCG05 expressing luciferase gene (Luciola red) under the control of xylose-inducible system (pBCG05-xylR-luciferase) | This study |
| pBCG07 | pCWU6 expressing TnaA under the control of xylose-inducible system combination with riboswitch E (pCWU6-xyR-E-tnaA) | This study |
| pFtsXFLAG | pCWU6 expressing H6-3xFLAG-FtsX | 28 |
| pBCG08 | pCWU6 expressing 3xFLAG tagged FtsX under the control of xylose-inducible system combination with riboswitch E (pCWU6-xyR-E-ftsX) | This study |
| pBCG10-∆lepB | Derivative of pCM-galK(ery), deletion vector of lepB | This study |
| pAN01 | Derivative of pCWU6 expression of LepB under the control of xylose-inducible system combination with riboswitch E | This study |