Fig 5.

Stability of viral Envs on ice. Virus particles with the indicated Envs were prepared from the supernatants of transfected 293T cells and incubated at 0°C for the period of time shown. The viruses were then pelleted, lysed, and analyzed by western blotting (A). The gp120/gp41 ratio (%) on the virus particles at each time point relative to that on a control virus not incubated at 0°C is reported (B). The results of a typical experiment are shown. The experiment was repeated with comparable results.