Phenotypes of HIV-1AD8 Env variants with changes near the gp120-gp41 cleavage site. Env processing, subunit association, gp120-trimer association, cell-cell fusion, and infectivity are shown for each HIV-1AD8 Env mutant, normalized to those values observed for the wt HIV-1AD8 Env. Cold sensitivity represents the pseudovirus infectivity after 24 h of incubation at 0°C, relative to that of the untreated virus. The 50% inhibitory concentrations (IC50 values) of the CD4-mimetic compound, BNM-III-170, are reported in µM. N/A, not available; ND, not determined. Values are colored according to the key, based on their fold increase or decrease compared to those of the wt HIV-1AD8 Env. The results shown are the means and SDs derived from at least two independent experiments.