TABLE 2.

Phenotypes of HIV-1_AD8 Envs with altered sites of N-linked glycosylation

Env	Virion Env a	Infectivity b	Resistance/sensitivity compared to wt AD8 Env c
			Cold	BNM-III-170	19b
N130E		+++	S	S
N136E		+++	RR	●
T138A		+++	RR	●
S143D		+++	R	●
S158E		–	ND	ND
N160E	d	+	●
N262E	+	+	RR	RR	SS
N295E	+++	+++	S	●	●
N301E	+++	++	SS	SS	S
T303K		++	SS
D325Q		+++	R	●
R327Q		+++
N332T	+++	++	S	S
N406E	+++	+++	●	●	●
N411E	+++	++	S	●	●
N448E	+	+++	●	●	●
N136E/D325Q		+++	RR	R
N130E/T138A		+++	R	●
T138A/S143E		++	R	S
T138A/S158E		–	ND	ND
Q3(V1)/N301E		++
Q3(V1)/T303K		++
N136E/N301E			●

^a The levels of Env on virus particles produced by transfection of 293T cells were estimated by pelleting and lysing the virus particles, followed by western blot analysis. +++, 75%–125% wt level; ++, 25%–74% wt level; +, 5%–24% wt level; –, <5% wt level.

^b The infectivity of recombinant viruses pseudotyped by the indicated Env was measured on TZM-bl target cells. +++, 75%–125% wt infectivity; ++, 25%–74% wt infectivity; +, 5%–24% wt infectivity; –, <5% wt infectivity.

^c The sensitivity/resistance of recombinant viruses pseudotyped by the indicated Env to cold exposure (0°C), the CD4mc BNM-III-170, and the 19b and 17b pNAbs, relative to that of the wt HIV-1_AD8, is reported. ●, wt level; R indicates resistance relative to the wt virus; S indicates sensitivity relative to the wt virus. An increase in the number of R or S symbols indicates a greater level of resistance or sensitivity, respectively, compared to the wild-type virus level. The values in the table that were not determined are designated ND or are left blank. The data shown are representative of results obtained in at least two independent experiments.

^d The virion Envs for the N160E mutant exhibit decreases in the ratio of mature gp120 to uncleaved (gp160) Env.