Skip to main content
. 2023 Sep 28;89(9):e00983-23. doi: 10.1128/aem.00983-23

Fig 2.

Fig 2

Comparison of relative transcription levels (RTLs) of sporulation-related genes between WT and mutant strains and yeast one-hybrid assay. (A–D) Comparison of RTLs of sporulation-related genes in ΔAomedA (A), AobrlA (B), AoabaA (C), and ΔAowetA mutants (D) versus WT strain. Error bars: SD from three replicates. Asterisk (A–D) indicates a significant difference between mutant and WT strain (Tukey’s HSD, P < 0.05). (E) Yeast one-hybrid assay of AoMedA, AoBrlA, AoAbaA, and AoWetA. pGADT7-Rec-p53/p53-AbAi as positive control and pABAI-AoMedA, pABAI-AoAbaA, and pABAI-AoWetA plus pGADT7 as negative controls. Yeast transformants were diluted in 0.9% NaCl, and serially diluted five times with equal volume for obtaining 100, 10−1, 10−2, 10−3, 10−4, and 10−5 dilutions.