Fig 7.

Detection of autophagy in WT and mutants (ΔAomedA and ΔAowetA). (A–C). Observation of autophagic vesicles of WT strain (A), ΔAomedA (B), and ΔAowetA (C) mutants stained by MDC (left panel) and observed by TEM (right panel). (D) Western blot analysis of autophagy in WT and mutant strains. Anti-SQSTM1/p62 antibody was used as an autophagosome marker and tubulin as the control. (E) Western blot analysis of peroxisome in WT and mutant strains. Anti-PEX5/PER3 antibody and anti-PEX7 antibody were used as peroxisome markers and tubulin as the control.