TABLE 4.
Primers used for Y1H and Y2H assays
| Primers | Sequence (5’−3’) | Description |
|---|---|---|
| AD-AoMedA F | ATATGGCCATGGAGGCCAGTATGGCAGCCTCCTACACCAA | Connected to pGADT7 vector |
| AD-AoMedA R | TATCGATGCCCACCCGGGTGGGACATCGAGTAGGTGTTGT | |
| AD-AoBrlA F | ATATGGCCATGGAGGCCAGTATGTCCCATCATAACATTCA | |
| AD-AoBrlA R | TATCGATGCCCACCCGGGTGATTTTCAAGCTTGATCCCCG | |
| AD-AoAbaA F | ATATGGCCATGGAGGCCAGTATGAGGAGCAGAACAAAGTC | |
| AD-AoAbaA R | TATCGATGCCCACCCGGGTGCTGCATATTGCCCATCCCAC | |
| ABI-AoMedA F | TTGAATTCGAGCTCGGTACCGATGAGAGATGGATGGTTTG | Connected to pABAI vector |
| ABI-AoMedA R | TCGACAGATCCCCGGGTACCGTTGCTGAGAGCCTGGGTGT | |
| ABI-AoAbaA F | TTGAATTCGAGCTCGGTACCCCGCATCGAGCAGAGAATTT | |
| ABI-AoAbaA R | TCGACAGATCCCCGGGTACCATTTGGGGATGATGTATATC | |
| ABI-AoWetA F | TTGAATTCGAGCTCGGTACCTAGGAAATTTCGCTGCATCG | |
| ABI-AoWetA R | TCGACAGATCCCCGGGTACCTTGAACTCAAGTTGCCCGGA | |
| AD-AoMedA F | ATATGGCCATGGAGGCCAGTATGGCAGCCTCCTACACCAA | Connected to pGADT7 vector |
| AD-AoMedA R | TATCGATGCCCACCCGGGTGGGACATCGAGTAGGTGTTGT | |
| BD-54g434 F | TGCATATGGCCATGGAGGCCATGGCTGCCGTTGCAAAACA | Connected to pGBKT7 vector |
| BD-54g434 R | GCAGGTCGACGGATCCCCGGGTCGTAAGCAGGTGGAGATG |