Fig 3.

Knockdown of Beclin1 reduces astrovirus replication. (A) Representative immunoblot and quantification of immunoblots for Beclin expression after siBeclin knockdown compared to siControl with statistical analysis by unpaired two-tailed t-test. (B) Genome copy number of human astrovirus in cell lysate at 24 hpi from HAstV-1-infected Caco-2 cells treated with either siBeclin or siControl with statistical analysis by unpaired two-tailed t-test. (C and D) Caco-2 cells were treated with siBeclin or siControl 24 h after plating, once cells had reached 70% confluency. At 48 h post-treatment with siRNAs, Caco-2 cells were infected with HAstV-1. At 24 hpi, Caco-2 cells were fixed. (C) EVOS microscope images show astrovirus capsid in green and nucleus (Hoechst) in blue. Quantification shows FFU with statistical analysis by unpaired two-tailed t-test. (D) EVOS microscope images show astrovirus double-stranded RNA (J2) in green and nucleus (Hoechst) in blue. Quantification shows FFU with statistical analysis by unpaired two-tailed t-test.