Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 27;54:82. doi: 10.1186/s13567-023-01213-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Generation and characterization of anti-bovine PD-1 rabbit mAbs. A Flow cytometric analysis using anti-bovine PD-1 rabbit mAbs (1D10F1 and 4F5F2) and anti-bovine PD-1 rat mAb (5D2). BoPD-1-myc cells were stained with the mAbs using serial dilutions (10 μg/mL to 100 pg/mL). Rat IgG_2a isotype control and rabbit IgG controls (1H4L and 4H1L) were used as negative controls. B, C Flow cytometric analysis of PD-1 expression in T-cell subsets of cattle (n = 3). Freshly isolated bovine PBMCs were stained with anti-bovine PD-1 rabbit mAbs (1D10F1 and 4F5F2) and anti-bovine PD-1 rat mAb (5D2). Rat IgG_2a isotype control and rabbit IgG control (1H4L) were used as negative controls. B Representative histograms of staining with anti-PD-1 mAbs (orange, green, or blue histograms) and matched negative controls (gray histograms) are shown. The gating strategy of this assay was provided as Additional file 3. C Percentages of PD-1⁺ cells in each T-cell subset in leukocytes from healthy cattle (n = 3). Bars indicate the group mean percentage. Significant differences between each of the two groups were determined using a Student’s t test. *p < 0.05. D BoPD-1-Ig was preincubated with anti-bovine PD-1 rabbit mAbs (1D10F1 and 4F5F2) and anti-bovine PD-1 rat mAb (5D2), and then reacted with BoPD-L1-EGFP cells. BoPD-1-Ig bindings were evaluated by flow cytometry. Rat IgG_2a and rabbit IgG controls (1H4L and 4H1L) were used as negative controls. Each curve represents the relative binding of BoPD-1-Ig preincubated with each antibody compared with the no-antibody control.