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. 2023 Sep 27;54:82. doi: 10.1186/s13567-023-01213-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Production of anti-PD-1 chimeric antibody, Boch1D10F1. A Schematic structures of plasmid vectors encoding Boch1D10F1 (pDC62c5-U533-Boch1D10F1, pDC61-Boch1D10F1, and pNC32c-U533-Boch1D10F1). The light chain consisting of a variable region (VL) and a constant region (CL). The heavy chain consisting of a variable region (VH) and a constant region (CH). mDHFR: modified dihydrofolate reductase, UCOE: ubiquitous chromatin opening element (UCOE), npt: neomycin-resistant gene. B Selection of CHO DG44 cell clones producing Boch1D10F1. The production capacity of Boch1D10F1 from each cell clone transfected with pDC62c5-U533-Boch1D10F1 (blue bars), pDC61-Boch1D10F1 (red bars), and pNC32c-U533-Boch1D10F1 (green bars) are shown. The production capacity of the highest producing clones for each vector are shown in the plot. C Expression of Boch1D10F1. Boch1D10F1 was expressed in 2L of shaking culture of a higher-producing cell line. The antibody production (left axis: gray bar) and the density of live and dead cells (right axis: black and white circles) were measured at 3- to 4-day intervals. D Purification of Boch1D10F1. Boch1D10F1 was purified from the supernatants of shaking cultures. Purified protein was confirmed by reducing and nonreducing SDS-PAGE. An uncropped gel image generated during the current study is provided in Additional file 4.