Table 1.

Binding affinity of anti-PD-1 mAbs to BoPD-1-His protein

Anti-PD-1 mAb	ka (1/Ms)	kd (1/s)	KD (M)
5D2**	1.84 × 104 ± 0.27	2.15 × 10–4 ± 0.44	1.22 × 10–8 ± 0.39
1D10F1	1.63 × 106 ± 0.16*	1.80 × 10–3 ± 0.06*	1.11 × 10–9 ± 0.07*
4F5F2	7.39 × 105 ± 0.90*	2.08 × 10–3 ± 0.28*	2.85 × 10–9 ± 0.50*

These values were measured on a Biacore X100 instrument

^*p < 0.05 (vs. 5D2; Tukey’s test)

^**The values for 5D2 were shown in a previous paper [10]