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. 2023 Sep 5;15(9):1877. doi: 10.3390/v15091877

Figure 2.

Figure 2

SDS-polyacrylamide gel electrophoresis showing the purification of N4S11-SC2 and immunoblotting of N4S11-SC2 protein against anti-HIS antibody and pooled serum samples from COVID-19 patients and negative control individuals. (A) SDS-polyacrylamide gel electrophoresis showing the purification of N4S11-SC2. FT = Flow-Through; L1 = First N4S11-SC2 Elution; L2 = Second N4S11-SC2 Elution; MW = PageRuler™ Unstained Broad Range Protein Ladder. (B) H1 and H2: First and second elutions of anti-HIS antibody; P1 and P2: First and second elutions of COVID-19 positive patient’s serum pools; N1 and N2: First and second elutions of negative pre-pandemic individuals’ serum pools; MW = Page Ruler™ Pre-stained Protein Ladder.