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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: J Heart Lung Transplant. 2023 Jun 12;42(10):1363–1377. doi: 10.1016/j.healun.2023.06.001

Figure 7.

Figure 7.

Impact of continuous immunosuppression on targeted IL-10 activation utilizing Ad-dSaCas9-VPR in the lung. (A, B) Rats were treated with two-gRNA adenoviruses under transient or continuous triple immunosuppression. Treated lungs were analyzed at day 14 for gene expression and transgene DNA in the lung tissue by qPCR (n=5 for each group). Median (interquartile range) are shown. (**: p ≤ 0.01; Mann-Whitney test). IS; immunosuppression. (C) Representative images of hematoxylin and eosin staining of the lungs 14 days after adenoviral vector administration. Scale bars, 100 μm. CD3-positive cell population in immunohistochemistry was measured using the Cytonuclear IHC algorithm in HALO image analysis platform. Median (interquartile range) were presented. Symbols indicate *: p ≤ 0.05 (Mann-Whitney test).