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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Neurobiol Aging. 2023 Jul 17;131:182–195. doi: 10.1016/j.neurobiolaging.2023.07.007

Figure 3. iPSC-Derived Neuron Cytoskeleton Assessment and Neurite Formation.

Figure 3.

A. Representative images of D70 neurons. Blue: Nuclear counterstain DAPI, Green: Actin filament stain Phalloidin, Red: β-tubulin 3 (TUBB3). Scale Bars: 125 μm C-F. Immunofluorescent assessment of actin filament organization and cytoskeleton pattern analysis of D70 neurons Ch1: Phalloidin, Ch3: TUBB3. C-E. Representative images and quantification of neurite formation recorded from D30 to D35 and presented as neurite length per surface area. The intersection of two masked neurites in an image is presented as branch points/cell body cluster count. Scale Bars: 150 μm. *: p-value<0.05, **: p-value<0.01, ***: p-value<0.001, ****: p-value<0.0001