Figure 5.

PASTE2 alignment of Stereo-seq data from an E14–16 Drosophila embryo from Wang et al. (2022). (A) Stereo-seq slices 7 and 8 with spots labeled by cell types annotated in Wang et al. (2022). (B) Spots from slices 7 and 8 that are included in the partial alignment computed by PASTE2. Spots selected by PASTE2 have similar proportions of cell types and spatial locations. (C) Optimal projection of slices 7 and 8 onto the same 2D coordinate system using the PASTE2 partial alignment. (D) PASTE2 3D reconstruction using all 16 slices from the Drosophila embryo.