Skip to main content
. 2023 Sep 14;14:1167965. doi: 10.3389/fimmu.2023.1167965

Figure 3.

Figure 3

GPI-scFv X5-transduced CEMss-CCR5 cells are resistant to iDC- or mDC-mediated trans-infection of HIV-1. (A) HIV-1 gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and HIV-1 AD8-captured iDCs. (B) HIV-1 gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and HIV-1 Bru-3-captured iDCs. (C) HIV-1 gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and HIV-1 AD8-captured mDCs. (D) HIV-1 gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and HIV-1 Bru-3-captured mDCs. (E) RLU detected in mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells trans-infected with or without HIV-1 pseudotyped AD8, Bru-3, Yu2, CNE3, CNE11, Con B, Con C or JRFL-captured iDCs at 48 hours post co-culture. RLU in HIV-1 pseudotyped AD8, Bru-3, Yu2, CNE3, CNE11, Con B, Con C or JRFL-captured iDCs alone were included for the comparison. (F) RLU detected in mock-, GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells trans-infected with or without HIV-1 pseudotyped AD8, Bru-3, Yu2, CNE3, CNE11, Con B, Con C or JRFL-captured mDCs at 48 hours post co-culture. RLU in HIV-1 pseudotyped AD8, Bru-3, Yu2, CNE3, CNE11, Con B, Con C or JRFL-captured mDCs alone were included for the comparison. Infections were performed in triplicate. The average values ± standard deviation (SD) are shown.