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. 2023 Sep 14;14:1167965. doi: 10.3389/fimmu.2023.1167965

Figure 5.

Figure 5

Trans-blockage of R5-tropic HIV-1 in iDCs by GPI-scFv X5. (A) HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and iDCs with captured HIV-1 MJ4 or iDCs with HIV-1 MJ4 alone. (B) HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between GPI-scFv X5 or AB65-transduced CEMss-CCR5 cells and iDCs with captured HIV-1 Yu-2 or iDCs with HIV-1 Yu-2 alone. (C) HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between CEMss-CCR5-GPI-scFv X5 or AB65 or CEMss-CCR5-CR2N cells and iDCs with captured HIV-1 Yu-2 or iDCs with HIV-1 Yu-2 alone. D and (E) HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9 and 12 post co-culture between CEMss-CCR5-GPI-scFv X5 cells and iDCs with captured HIV-1 Yu-2 (D) or AD8 (E) in a regular plate (open squares) versus a transwell plate (closed squares) or iDCs with HIV-1 Yu-2 (D) or AD8 (E) alone in a regular plate. Infections were performed in triplicate. The average values ± standard deviation (SD) are shown.