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. 2023 Sep 14;14:1167965. doi: 10.3389/fimmu.2023.1167965

Figure 6.

Figure 6

GPI-scFv X5 protects CD4 T cells in cis but not in trans when displayed on CD8 T cells. (A) Expression of CD4+ (left panel) or CD8+ (right panel) and GFP in human primary CD4+ T cells and CD8+ T cells transduced with recombinant lentiviral vectors containing pRRL-GPI-scFv X5-2A-GFP, respectively, after a single round transduction. (B) HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9, and 12 post culture of CD4+, GPI-scFv X5 CD4+ or co-cultured with CD8+, or GPI-scFv X5 CD8+ infected with HIV-1 NL4-3. (C) Two additional donors, Donor 2 (blue) and Donor 3 (purple), were evaluated for GPI-scFv X5 modified CD8 T cell mediated trans protection of CD4 T cells. HIV-1 Gag p24 in the culture supernatants collected at days 3, 6, 9, 12, and 15 post co-culture of CD4+ with unmodified CD8+ (solid line) or GPI-scFv X5 CD8+ (dotted line) infected with HIV-1 NL4-3. Infections were performed in triplicate. The average values ± standard deviation (SD) are shown.