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Experimental design. A, Monkeys received AAV-PSAM4 vector injections into the striatum (n = 2 monkeys) and primary motor cortex (MI; n = 1). B, [18F]ASEM-PET was performed to visualize PSAM4 expression in vivo. C, After administering uPSEMs, activation mapping using [18F]FDG-PET imaging allowed the relationship between agonist type/dose and activation strength to be determined. D, Pharmacological MRI was used to measure the time course of chemogenetically induced activity changes.
