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. 2023 Sep 27;43(39):6592–6608. doi: 10.1523/JNEUROSCI.2206-21.2023

Figure 9.

Figure 9.

Fyn expression in iBottg/+ spinal cords is concentrated in NKX2.2− BoNT/B-expressing OLs. A, Immunolabeling for Fyn and NKX2.2 in iBottg/+ dorsal horn, inset shows overlay with GFP. B, Immunolabeling for Fyn and NKX2.2 iBot+/+ dorsal horn, inset shows enlarged view of control premyelinating OL. C, GFP, NKX2.2, and Fyn labeling in iBottg/+ dorsal corticospinal tract. D, Western blot analysis for total Fyn (green) and GAPDH (magenta) from P14–P15 iBot+/+ and iBottg/+ spinal cord lysates. E, Quantification of total Fyn signal normalized to GAPDH from iBot+/+ (8.99 × 103 ± 705.49 a.u.) and iBottg/+ (9.18 × 103 ± 907.86 a.u.) spinal cord lysates (Welch’s two sample t test, t(6.06) = −0.168, p = 0.872, n = 5 iBot+/+ and 4 iBottg/+ mice). Scale bar = 25 µm (A–C).