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. 2023 Sep 28;18(9):e0291242. doi: 10.1371/journal.pone.0291242

Fig 5. Effect of essential oils on the eye area of flies.

Fig 5

Figures A through E show the eyes of flies from different groups. The Canton S group (A) had a normal eye, while the negative control group (B) with Eiger protein overexpression had a malformed eye, representing the GMR-GAL4 > UAS-Eiger strain. Experimental groups of GMR-GAL4 > UAS-Eiger flies treated with the essential oils at 0.25% v/v are shown in Figures C to E (C: copaiba EO, D: ginger EO, and E: lavender EO). Images were taken with a stereomicroscope at 3X magnification. Measurements taken with ImageJ show that only lavender essential oil was able to ameliorate the inflammation of the fly eye and slightly restore its area (F). Statistical significance was determined by one-way ANOVA test (n = 60, **** p<0.0001), with graphics demonstrating mean ± standard error of the mean. This test was performed on three independent triplicates.