Figure 5.

Slot blots of clones from the second immunoprecipitation. Slot blots of 100 ng of insert cDNA from each unique clone isolated by library screening, LPS activated THP-1 library, with RT–PCR of hnRNP A2-associated polysomal RNA were probed at a concentration of 2 × 10⁶ c.p.m./ml and used for autoradiography on the same piece of film. The probe was generated by ³²P random primer labeling of the RT–PCR of a set of second immunoprecipitation with the indicated antisera from LPS + THP-1 polysomes. Asterisks indicate positive clones as determined by densitometery. The identity of each clone is listed in Table 2.