Table 1. Clones from the immunoprecipitation of leukemic polysomes.
| Blot position | Clone name | Nonamer (UUAUUUAU/AU/A) | Pentamer (AUUUA) | Poly(U) tract(s) |
|---|---|---|---|---|
| A-1 | EPA glycoprotein | – | – | – |
| A-2 | Vimentin | – | + (1) | + |
| A-3 | Chrom 6p22.3–24.3 | |||
| A-4 | Novel sequence | |||
| A-6 | Heparin sulfate proglycan core | – | + (6) | + |
| A-7 | Ribosomal protein L44 | – | – | – |
| A-8 | Novel sequence | |||
| A-9 | Ribosomal protein L18a | – | – | – |
| A-11 | KM 102-derived reductase-like factor | + (1) | + (2) | + |
| A-12 | Novel sequence | |||
| B-1 | Chromo Xp11.23–11.3 | |||
| B-2 | Chromo Xq25 | |||
| B-3 | Cyclooxygenase I | – | + (1) | + |
| B-4 | sec61 homolog | – | – | + |
| B-6 | SOM172 (phospholipase C B3) | – | – | + |
| B-7 | PEA-15 astrocytic phosphoprotein | – | – | + |
| B-8 | Cathepsin B | – | – | + |
| B-9 | Inducible poly(A)-binding protein | – | + (1) | + |
| B-11 | Chromo 22q12 matches EST | |||
| B-12 | Adenylyl cyclase associated protein | – | – | + |
| C-1 | Novel sequence | |||
| C-2 | GTF2I (transcription factor) | – | + (1) | + |
| C-3 | β-actin | – | – | + |
| C-4 | Ribosomal protein L30 | – | – | – |
| C-6 | Novel sequence | |||
| C-7 | Heme oxygenase (decycling) 1 | – | + | |
| C-8 | Novel sequence | |||
| C-9 | Cytochrome oxidase subunit II | – | – | – |
| C-11 | TAGLN2 | – | + (3) | – |
Clones identified from the first immunoprecipitation. List of the 29 clones from the first immunoprecipitate examined by slot blotting, along with the presence or absence of the indicated 3′ UTR cis-elements, which are defined as follows: 3′ UTR, the region of the message from the stop codon to the start of the poly(A) tail; pentamer, the sequence AUUUA (ATTTA for cDNA); nonamer, the sequence UUAUUUAU/AU/A (TTATTTAT/AT/A in cDNA); and poly(U) tract, UUUU occurring four times or UUUUU occurring twice. The number in parentheses in the pentamer column indicates the number of pentamers present in the 3′ UTR. The blot position corresponds to the location of the clone on the slot blot in Figure 4.