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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: FASEB J. 2023 Oct;37(10):e23198. doi: 10.1096/fj.202300386RR

Figure 6. DOCK3 interacts with insulin signaling protein, SH3 domain-containing 1 (SORBS1).

Figure 6.

A. DOCK3-SORBS1 yeast-2-hybrid neuromuscular cDNA screening library strategy. B. Selection guide of DOCK3-SORBS1 yeast-2-hybrid amino acid selection. C. Positive interaction of DOCK3-C-terminus and SORBS1 cDNA shown with yeast selective growth. D. DOCK3-SORBS1 co-Immunoprecipitation in HEK293T cells. Immunoprecipitation (IP) performed with DOCK3-GST and immunoblotting (IB) against SORBS-FLAG. E. GST-Pulldown domain constructs shown indicating domains of full-length Dock3 and GST tagged PXXP motif. F. DOCK3-SORBS1 GST-Pulldown immunoblots showing the interaction between recombinant DOCK3 and SORBS1 directly interacting.