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Competitive binding of 12 with antagonist, XAC and A2AAR-selective agonist, CGS21680 in HEK293 cells expressing hA2AAR. Fluorescence intensity was measured using a Beckman Coulter CytoFLEX B4-RO-VO System with a 638-nm laser and a 660/20 bandpass filter. A Ki of 53 ± 6 nM (N = 2) was calculated for XAC and 783 ± 52 nM (N = 2) for CGS21680 based on a Kd of 222 nM for 12 at hA2AAR
