Figure 3.

Effect of melatonin (MLT) and 5-fluorouracil (5-FU) on the myosin light-chain kinase (MLCK) signaling pathway in gastric cancer (GC) cells. (A) GC cells were cultured and treated with the MLT and 5-FU combination for 48 h. (B-E) The expression level of MLCK and the phosphorylation level of MLC were determined using western blotting, which were significantly downregulated by the combination treatment. The experiments were performed three times to ensure accuracy and consistency. One-way ANOVA with Tukey's multiple comparison test was used for statistical analysis. Data are presented as mean ± SD. And statistical significance is indicated using asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).