Figure 4.

Localization of the ORN to CD8+ T-cells, overlap of ORN with TNBC anti-PD-L1 therapy response gene signature, and predicted opioid downregulation of anti-PD-L1 response. (A) Spearman correlation plot between turquoise network expression (“ORN”) and CIBERSORT estimate of CD8⁺ T-cell proportion for each FUSCC patient sample. (B). Fisher’s exact test odds ratio and -log10 Bonferroni p-value estimating ORN significant overlap with the anti-PD-L1 gene signature in peripheral and TNBC intratumoral T-cells. (C) A subset of the ORN is pictured here, with large labeled nodes denoting genes whose RNA expression is modulated in immunotherapy responders in TNBC. (D) For the 72 genes that overlap between the morphine and anti-PD-L1 response signatures, the change in RNA expression for each gene in response to morphine (y-axis) and immunotherapy (x-axis) is respectively illustrated. Each point is one of the overlapping genes.