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Effects of CBD on the expression of tight junction proteins. A Densitometry and B Western blots of tight junction proteins after incubation with CBD for 48 h (one-way ANOVA, n = 6; *p < 0.05). Immunofluorescent staining of C claudin-1 (green) and Zonula occludens 1 (ZO-1; red), D claudin-4 (green) and claudin-5 (red) after 48 h of incubation. Nuclei were stained in blue (DAPI; scale bar: 20 μm; n = 4; representative images).
