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Specific TNF receptor expression after co-incubation with 1000 U/mL TNFα and CBD. A Densitometry and B Western blots of TNFR-1 and TNFR-2 after 48 h of incubation with TNFα and CBD (n = 9–12). Immunofluorescent staining of C TNFR-1 (green), D TNFR-2 (green), nuclei were stained in blue (DAPI; scale bar: 20 μm; n = 4; representative images). Z-stack images of E TNFR-1(green) and F TNFR-2 (green) (DAPI (blue); scale bar: 20 μm; n = 3; representative images).
