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. 2023 Sep 25;19(15):5004–5019. doi: 10.7150/ijbs.86717

Figure 3.

Figure 3

Fecal transplantation with microbiota from HFD-fed mice enhances inflammation-driven CAC. (A) Experimental design for FMT treatment of CD-fed and HFD-fed mice in a CAC mouse model. (B) Weight changes in CD-FMT and HFD-FMT mice before sacrifice. (C) Macroscopic polyps (arrows) were identified in the distal and mid colons. (D) Day-60 weight alterations in CD-FMT and HFD-FMT mice before sacrifice. (E-F) The number (E) and maximal cross-sectional area (F) of macroscopic polyps were quantified. (G) Representative pictures of H&E staining in each group. Scale bar = 100 μm. (H-K) Histopathological analysis of colon inflammation (H), and area associated with disease (I), hyperplasia (J), and dysplasia (K) in CD-FMT and HFD-FMT mice. (L) Quantitative analysis of pathological score was calculated according to the following criteria: 0, normal; 1, LGD; 2, HGD; and 3, carcinoma. H&E, hematoxylin and eosin; LGD, low-grade dysplasia; HGD, high-grade dysplasia. CD-FMT, n = 8; HFD-FMT, n = 8. *, P < 0.05; **, P < 0.01; ***, P < 0.001.