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. 2023 Sep 25;19(15):5004–5019. doi: 10.7150/ijbs.86717

Figure 7.

Figure 7

HFD promotes NLRP3-associated pro-inflammatory mediator expression by limiting butyrate synthesis and secretion. (A) Representative immunofluorescence staining of Caspase-1 in colon tissues of mice with CD, HFD, H/F+water, and H/F+butyrate treatment. Scale bars = 100 μm. (B) Quantification of Caspase-1 expression by calculating mean density (integrated density/specimen area). (C) Representative immunofluorescence staining of IL-1β in colon tissues (D) Quantification of IL-1β expression by calculating mean density (integrated density/specimen area). (E) The expression levels of NLRP3, cleaved caspase-1 (P20), and mature IL-1β (P17) were detected after treatment with different concentrations of butyrate. (F) The bands were quantified. H/F, HFD-FMT. Buty, butyrate. L/A, LPS/ATP. ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001.