Figure 3.

Srsf1 deficiency causes the loss of germ cells. (A) MVH (a marker of germ cells, purple), SOX9 (a marker of Sertoli cells, red) and PLZF (a marker of undifferentiated spermatogonia, green), immunofluorescence analysis of the Srsf1^WT and Srsf1^cKO 8-week-old male mice. Scale bar: (top) 50 μm; (bottom) 20 μm. (B) Quantification of the SOX9 positive signal cells in the seminiferous tubules of the Srsf1^WT and Srsf1^cKO mice. (C) Quantification of the PLZF positive signal cells in the seminiferous tubules of the Srsf1^WT and Srsf1^cKO mice. (D) SYCP3 (red) and γH2AX (green) immunofluorescence analysis of the Srsf1^WT and Srsf1^cKO 8-week-old male mice. Scale bar: (top) 50 μm; (bottom) 20 μm. (E) Quantification of the SYCP3 positive signal cells in the seminiferous tubules of the Srsf1^WT and Srsf1^cKO mice. (F) Statistics of the ratio of SYCP3-positive tubule cross-sections in Srsf1^WT and Srsf1^cKO mice. (G) Srsf1^WT and Srsf1^cKO mice chromosome spreads of spermatocytes were immunostained with antibodies against SYCP3 (green) and γH2AX (red). γH2AX marked DSB in spermatocytes(8-weekold). Scale bar: 10 μm. In this part, at least 3 mice of each genotype were used for the analysis.