Figure 4.

SRSF1 depletion results in the apoptotic death of germ cells. (A) TUNEL immunofluorescence staining of the testes of Srsf1^WT and Srsf1^cKO. (Scale bar: 50 μm) Green: TUNEL positive signal; Blue: DAPI. At least 3 mice (8-week-old) of each genotype were used for analysis. (B) Number of TUNEL positive cells per tubule section. (C) Quantification of TUNEL positive seminiferous tubules (8-week-old). (D) PLZF (green) and MVH (red) immunofluorescence analysis of the Srsf1^WT and Srsf1^cKO male mice at P8, P10 and P12. Scale bar, 20 μm. In this part, at least 3 mice of each genotype were used for the analysis. (E) Quantification of the PLZF positive signal cells in the seminiferous tubules of the juvenile Srsf1^WT and Srsf1^cKO mice. For each time point, at least 3 mice of each genotype were used for the analysis. Data are presented as the mean ± SEM. P<0.05(*), 0.01(**) or 0.001(***). (F) Quantification of the germ cells in the seminiferous tubules of the juvenile Srsf1^WT and Srsf1^cKO mice. For each time point, at least 3 mice of each genotype were used for the analysis. Data are presented as the mean ± SEM. P<0.05(*), 0.01(**) or 0.001(***).