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. 2023 Sep 11;19(15):4883–4897. doi: 10.7150/ijbs.83474

Figure 7.

Figure 7

The expressions of key SRSF1-binding genes involved in the spermatogenesis change after Srsf1 KO. (A) Venn diagram showing the overlapped genes between differentially expressed genes and SRSF1-binding genes. (B) Correlation analysis between the RNA-seq and LACE-seq. GO analysis of the significantly upregulated genes and SRSF1-binding genes. (C) Correlation analysis between the RNA-seq and LACE-seq. GO analysis of the significantly downregulated genes and SRSF1-binding genes. (D) Quantitative RT-PCR validation of the expression of genes involved in (B). β-actin was used as the internal control. Data are presented as the mean ± SEM. P<0.05(*), 0.01(**) or 0.001(***). (E) Quantitative RT-PCR validation of the expression of genes involved in (C). β-actin was used as the internal control. Data are presented as the mean ± SEM. P<0.05(*), 0.01(**) or 0.001(***).