Figure 8.

Srsf1 is required for normal splicing of functional genes during spermatogenesis. (A) The five different types of alternative splicing (AS) events. The numbers of abnormal AS events were counted between Srsf1^WT and Srsf1^cKO testes by rMATS software. (B) A magnified view showing RNA-seq signals of the selected candidate genes. IgG, immunoglobulin G. Semiquantitative RT-PCR analysis of AS patterns of the changed spliced genes in Srsf1^WT and Srsf1^cKO testes at P10. (C) Venn diagram shows the correlation between SRSF1-binding genes and AS genes. (D) GO analysis of the AS genes and SRSF1-binding genes. (E) A magnified view showing RNA-seq and LACE-seq signals of the Stra8 genes. IgG, immunoglobulin G. Semiquantitative RT-PCR analysis of AS patterns of the changed spliced genes in Srsf1^WT and Srsf1^cKO testes at P10. PCR primers are listed in Table S1.