Table 1.

Chemical shifts and line widths of the paramagnetic ${}^{\mathrm{1}}$ H NMR spectrum of oxidized MitoNEET, recorded at 600 MHz, and the proposed signal assignment.

Signal	283 K	293 K	$\mathrm{\Delta }\mathit{\nu }\mathrm{293}$  K	Proposed
	(ppm)	(ppm)	(Hz)	assignment
A	53.8	54.4	2700	His-87 H ${}^{\mathit{\delta }\mathrm{2}}$
B ${}_{\mathrm{exch}}$	46.8	47.3	2500	His-87 H ${}_{\mathrm{N}}^{\mathit{\epsilon }\mathrm{2}}$
C	43.4	43.9	2300	Cys-83 H ${}^{\mathit{\beta }\mathrm{3}}$
D	34.1	34.3	1800	Cys-74 H ${}^{\mathit{\beta }\mathrm{2}}$
E	25.7	25.9	1500	Cys-72 H ${}^{\mathit{\beta }\mathrm{3}}$
H ${}_{\mathrm{N}\mathrm{exch}}$	13.6	13.5	150
H ${}_{\mathrm{N}\mathrm{exch}}$	12.08	12.02	200
H ${}_{\mathrm{N}\mathrm{exch}}$	11.39	11.35	120
F	10.52	10.61	250	Cys-74 ${\mathrm{H}}^{\mathit{\alpha }}/$  His-87H ${}^{\mathit{\beta }\mathrm{2}}$
H ${}_{\mathrm{N}\mathrm{exch}}$	10.15	10.15	70	Glu-38 H ${}_{\mathrm{N}}$