TABLE 4.
Comparison of RT susceptibility data obtained with recombinant viruses with sequences encoding RT only or with recombinant viruses with sequences encoding PR and RTa
| Patient no. | Construct | IC50 (μM) (fold resistanceb)
|
||||
|---|---|---|---|---|---|---|
| AZT | 3TC | ddI | Loviride | Nevirapine | ||
| 3 | ΔRT | 1.91 (44) | >100 (>67) | 9.15 (2) | 2.41 (50) | 16.4 (214) |
| ΔPR/RT | 4.54 (49) | >100 (>13) | 12.3 (2) | 3.49 (46) | 94.7 (622) | |
| 11 | ΔRT | 0.16 (13) | >100 (>13) | 10.1 (2) | 0.09 (2) | NDc |
| ΔPR/RT | 1.49 (16) | >100 (>13) | 13.1 (2) | 0.10 (1) | 0.53 (3) | |
| 12 | ΔRT | 0.10 (2) | 9.25 (1) | 7.02 (3) | 0.08 (1) | ND |
| ΔPR/RT | 0.09 (1) | 9.76 (1) | 9.52 (1) | 0.14 (2) | 0.31 (2) | |
| 13 | ΔRT | 1.51 (19) | >100 (>48) | 56.4 (4) | 0.08 (1) | 0.28 (2) |
| ΔPR/RT | 1.87 (17) | >100 (>42) | 53.7 (5) | 0.08 (1) | 0.14 (1) | |
| 14 | ΔRT | 11.6 (142) | >100 (>48) | 47.4 (4) | 0.18 (3) | 1.08 (8) |
| ΔPR/RT | 6.68 (60) | >100 (>42) | 36.1 (4) | 0.21 (3) | ND | |
RT susceptibility data for chimeric viruses with RT only (ΔRT) were compared with similar data obtained with recombinant viruses with PR and RT (ΔPR/RT). Plasma samples from 35 patients (70 recombinant viruses) were investigated. Data for five samples with profiles indicating resistance to NRTI and NNRTI are presented. Resistance data are given as IC50s and as fold resistance.
Fold increase in the mean IC50 relative to the mean IC50 for the wild type. The mean IC50s for both patient and wild-type viruses were derived from two to four separate susceptibility determinations by the MT4-MTT assay.
ND, not determined.