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Engineering a DC-targeting anthrax protective antigen. (A) Schematics for the protein ligation of mPAC83 and an anti-XCR1 scFv: thiol-conjugation reaction with peptide 1a, followed by sortase-mediated ligation with the scFv (scFv-LPSTG2-H6). (B, C) Coomassie-visualized SDS-PAGE gels of fractions obtained from size-exclusion (HiLoad 16/600 Superdex 200) and anion-exchange (HiTrap Q HP) chromatography. (D) Schematic of the sortase-mediated ligation reaction for scFv and peptide 1b.
