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. 2023 Sep 14;9(9):1835–1845. doi: 10.1021/acscentsci.3c00625

Figure 5.

Figure 5

Targeting XCR1 facilitates trafficking to lymph nodes and antigen-presenting cells. (A,C) Time-course analysis of AF647 signal in mouse organs: two mice per time point for mPAC and scFv; one mouse per time point for scFv-mPAC. Mice were treated with 1 nmol of an AF647-labeled construct: mPAC, anti-XCR1 scFv, or anti-XCR1 scFv-mPAC. The constructs were subcutaneously (sc) administered over two equal volume injections, one on each side of the tail base (n = 5 mice per group for mPAC and scFv; n = 3 mice for scFv-mPAC). (A) Representative images obtained after 2 h using an in vivo imaging system (IVIS), showing AF647 signal from resected lymph nodes (inguinal and axillary), spleen, and liver. Data represent the mean of whole-organ radiant efficiency ± s.d. (B) Quantification of the AF647 signal after 2, 24, and 48 h. (C) Flow cytometry analysis of the AF647 signal after 2 h in single-cell splenocyte populations, including CD8+ dendritic cells (CD11c+CD8+), medullary macrophages (CD11b+F4/80+), and T and B cells (CD3+B220+). Data represent the mean of AF647+ cells ± s.d. All data are representative of two independent experiments.