Table 2.

Examples of experimental types which could be applied as validation sub-studies for methods used to evaluate vector control products

Method purpose	Example/outcome	Sub-studies	Analytical parameters
Measure immediate lethality effects	• KD and 24 h mortality	• Define test conditions (P) • Replication • Comparison*	• Robustness (P) • Precision
Quantify downstream outcomes	• Delayed mortality (72–96 h) • Impacts on fecundity	• Define test conditions (P) • Replication • Comparison*	• Robustness (P) • Precision
Monitor success/failure of an outcome during exposure	• Blood-feeding success	• Define test conditions (P) • Replication • Comparison*	• Robustness (P) • Precision
Measure induced sterility	• Oviposition inhibition	• Define test conditions (P) • Replication • Comparison*	• Robustness (P) • Precision
Characterize behavioural responses during- and post-exposure	• Proportion of net contact time	• Define test conditions (P) • Replication • Comparison*	• Robustness (P) • Precision
Monitor durability	• Efficacy of ITNs after 3 years deployment • Duration of IRS efficacy	• Define test conditions (P) • Linearity and reportable range • Comparison*	• Robustness (P) • Linearity • Range • Precision
Evaluate combination products	• ITN with different concentration/s of AI/s on different parts of the net	• As per method purpose for each combination	• As per method purpose for each combination

P Preliminary development phase

^*If applicable