Fig. 5.

SQLE Knockdown inhibited T24 and 5637 cell cycle and promoted cell apoptosis. (A) Flow cytometry was used to detect the proportion of G1, S, and G2/M phase cell population in T24 and 5637; (B) Flow cytometry was used to detect the early apoptosis and late apoptosis cell population in T24 and 5637; (C, D) The western blot assay was used to evaluate the expression of cell cycle and cell apoptosis related genes; (E–G) Representative images of JC-1 immunofluorescence and the ratio of red to green fluorescence showed a decrease in the mitochondrial membrane potential. Data are presented herein as the from three independent experiments. Data represent the mean ± SD of three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001 versus the vector group, ns, not significant)