Fig. 5.

The effect of oxamate plus CAR-T was suppressed by CD73 overexpression in GSCs. (A) CD73 expression in GBM patients using single-cell RNA-seq. (B) CD73 expression by IF detection in the xenografts of nude mice. Scale bar represents 20 μm. (C) CD73 expression in GSCs was measured after vector or CD73 cDNA transfection. (D, E) 51 A GSCs transfected with vector or CD73 cDNA were orthotopically inoculated into nude mice to establish glioma model, then sodium oxamate and CAR-T were injected for antitumor therapy. N = 5. (D) Survival of nude mice was recorded. (E) Tumor infiltrating CD8 + T cells was stained 28 days after GSCs implantation. Scale bar represents 50 μm. (F) GSCs were prelabeled by CFSE, then co-cultured with CD8 + T cells at effector/target ratio of 5 : 1 for 16 h with or without 10 mM sodium oxamate. Dead cells were stained with 7-AAD and detected by flow cytometry. Double positive cells were dead tumor cells. (G) The concentration of IFN-γ and granzyme B were measured in co-cultured medium. *P < 0.05, **P < 0.01