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. 2023 Sep 29;24:237. doi: 10.1186/s12931-023-02528-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mechanical stretching for 48 h induced the apoptosis ABSCs. (a) Cell apoptosis was detected by an annexin V FITC/ propidium iodide (PI) staining and flow cytometry analysis. Live cells (Q4) were stained negative for both annexin V and PI. Late apoptotic cells (Q2)were stained positive for both annexin V and PI. Early apoptotic cells (Q3) were stained positive for annexin V and negative for PI. Necrotic cells (Q1) were stained negative for annexin V and positive for PI. The apoptosis rate included Q2 and Q3. (b) Data are presented as the mean ± SD. **P < 0.01 compared with the control group. (c) Mechanical stretching for 48 h induced the activation of caspase 3 enzyme. Data are expressed as the mean ± SD from at least three experiments. ***P < 0.001 compared with the control group