Table 2.

BoGH13A_Sus CBM98 and CBM98–CBM48 ITC data

Construct	Ligand	Kd (μM) $\pm$ SD	N $\pm$ SD
CBM98	maltoheptaose	32 $\pm$ 9.8	0.8 $\pm$ 0.16
	α-cyclodextrin	42 $\pm$ 13	0.75 $\pm$ 0.2
	β-cyclodextrin	26 $\pm$ 3.8	0.81 $\pm$ 0.03
	maltotriose	346 $\pm$ 46	1*
	AP potato	71 $\pm$ 3	1.01 $\pm$ 0.01#
CBM98 W92,98A	maltoheptaose	NB	NB
	α-cyclodextrin	NB	NB
	β-cyclodextrin	NB	NB
	maltotriose	NB	NB
	AP potato	NB	NB
CBM98–CBM48	maltoheptaose	33 $\pm$ 9.9	0.74 $\pm$ 0.26
	α-cyclodextrin	43 $\pm$ 18	0.58 $\pm$ 0.3
	β-cyclodextrin	21 $\pm$ 1.4	0.77 $\pm$ 0.09
	maltotriose	360 $\pm$ 102	1*
	AP potato	976 $\pm$ 23	0.99 $\pm$ 0.03#
CBM98–CBM48 W92,98A	maltoheptaose	NB	NB
	α-cyclodextrin	NB	NB
	β-cyclodextrin	NB	NB
	maltotriose	NB	NB
	AP potato	NB	NB

NB stands for no binding

*Indicates that N was set to 1 because of low-affinity binding

^#Indicates that substrate concentration was adjusted to achieve an N of 1 according to [73]