Table 5.
LC-tandem-MS identification of tocopherols, tocotrienols, and γ-oryzanol compounds content in HRF and HRHF extracts.
| Compound name | Ret. time (min) | (M + H)− | Base beak m/z | mg 100 g−1 DW | Antioxidant activity | ||
|---|---|---|---|---|---|---|---|
| HRF | HRHF | ||||||
| 1 | α-Tocopherol | 0.49 | 429.38 | 237 | 3.63 ± 0.33a | 3.97 ± 0.17a | Strong (56, 57) |
| 2 | α-Tocotrienol | 1.52 | 423.33 | 423 | 1.76 ± 0.16a | 0.94 ± 0.04b | Highly strong (56, 57) |
| 3 | γ-Tocopherol | 3.71 | 415.36 | 239 | 2.11 ± 0.19a | 1.54 ± 0.07b | Active (56, 57) |
| 4 | γ-Tocotrienol | 4.95 | 395.30 | 195 | 6.97 ± 0.63 | 0.0 | Active (56, 57) |
| 5 | δ-Tocopherol | 5.82 | 401.34 | 179 | 3.24 ± 0.29a | 1.16 ± 0.05b | Active (56, 57) |
| 6 | δ-Tocotrienol | 6.42 | 409.30 | 199 | 5.52 ± 0.50 | 0.0 | Active (56, 57) |
| 7 | γ-Oryzanol | 7.39 | 601.90 | 601.90 | 24.70 ± 1.23 | 0.0 | Strong (56, 57) |
Each data is the average ± standard deviation across three distinct replications. According to student’s two-sample t-test, the letters in the same row display a significant difference at p ≤ 0.05.