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. 2023 Sep 15;13:1257897. doi: 10.3389/fcimb.2023.1257897

Figure 1.

Figure 1

Control of SAP activity in C. auris by the Ras/cAMP/PKA signaling pathway. (A–C) A comparison of SAP activities of C. auris wild-type and Ras/cAMP/PKA mutant strains at various temperatures. Cells of the wild-type and mutants tpk1Δ tpk2Δ (YSBA24), cdc25Δ (YSBA61), cyr1Δ (YSBA21), ras1Δ (YSBA41), gpa2Δ (YSBA39), ras1Δ gpa2Δ (YSBA68), and gpr1Δ (YSBA46) were cultured overnight, washed twice with deionized water (dH2O), and resuspended in 1 mL dH2O. We spotted 3 µL of the resuspended cell onto solid YCB-BSA media and incubated them for 3 days at (A) 30°C, (B) 37°C, and (C) 42°C. The values indicated below each figure represent the average halo thickness. Three biologically independent experiments were performed and one representative data are shown here. Error bars indicate standard deviation. Statistical analysis was performed using one-way ANOVA with Bonferroni’s multiple-comparison test (*P < 0.05; ****P < 0.0001).