| Parameter (with description) | Value used in simulations |
|---|---|
| Simulation timestep (dt) (in second) | 10−7 |
| Crowder Temperature Tc relative to Troom = 298.15K | 1 |
| Viscosity (in Pa · s) | 0.03 |
| Simulation box length (in nm) | 860 (for the actual system) 400 (for the monomeric system to obtain Kd) |
| Number of rod proteins | 1170 (for the actual system) 200 (for the monomeric system) |
| Number of hexamers | 390 (for the actual system) 200 (for the monomeric system) |
| Number of binders on each hexamer | 6 |
| Number of binders on each rod | 2 |
| Volume fraction of ribosomes (crowders) | 0.0 – 0.5 |
| Diameter of rod (in nm) | 23.4 |
| Diameter of hexamer (in nm) | 12.6 |
| Diameter of ribosome (in nm) | 30.0 |
| Maximum binding distance dbind (in nm) | 2.3 |
| Rate constants for dynamic unbinding (koff) (in s−1) | 26 |
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