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[Preprint]. 2023 Sep 19:2023.09.18.558356. [Version 1] doi: 10.1101/2023.09.18.558356

Extended Data Figure 9: Exploring the sequence and structural determinants for activating Hsp70.

Extended Data Figure 9:

a, AlphaFold2 predictions of the top 41 JDMs in complex with Hsp70. JDM37 is solid green while the remaining JDMs are transparent. b, Multiple viewpoints of either native J-domain:Hsp70 (cyan:yellow) (PDB: 5NRO) or JDM37:Hsp70 complex (blue:green). c, Sequence alignment of native J-domain from DnaJ versus JDM37. d, Clustal alignment of all human native J-domains. e, Various maps of either native J-domain:Hsp70 (top) or JDM37:Hsp70 (bottom) complex. Left: ribbon representation, Middle: electrostatic map of native J-domain or JDM37, Right: electrostatic map of Hsp70.