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. 2023 Oct 2;133(19):e162731. doi: 10.1172/JCI162731

Figure 3. MS rAbs bind to the surface of PLP1-transfected cells.

Figure 3

(A) Live-cell binding of myelin-specific rAbs (MS#30, ON#49, and ON#34) to CHOK1 cells transfected with MOG or PLP1 expression vector plasmid DNA at 24 hours after transfection. Live-cell staining with MOG-specific 8-18C5 mAbs (10 µg/ml) or PLP1-specific O10 mAbs (1:2 dilution of hybridoma supernatant) serve as positive controls. Scale bars: 25 µm. (B) Live-cell immunofluorescence (×600) of IC and myelin-specific MS rAbs (MS#30, ON#49, ON#34, and MS#11) in HEKE cells expressing PLP1. Human rAb binding is shown in green (rAb) and staining with an intracellular epitope-specific PLP1 mAb (plp1c or AA3) is shown in red (PLP1).