Skip to main content
. 2023 Oct 2;133(19):e162731. doi: 10.1172/JCI162731

Figure 4. Myelin-specific MS rAbs do not bind myelin or mediate oligodendrocyte cell death in PLP1-null animals.

Figure 4

(A) Cerebellar sections from WT (PLPy/+) and null (PLPy/–) male EGFP-PLP knockin mice were stained with myelin-specific MS rAbs (MS#30, ON#49, ON34), anti-PLP1 mAb AA3 (PLP1), and anti-MOG 8-18C5 mAbs. Scale bars: 50 µm. (B) EGFP+ immunofluorescence (×100) in murine brain following ICI of MS#30 or ON#34 rAbs with HC into male WT (PLPy/+) and hemizygous null (PLPy/–) EGFP-PLP knockin mice. Quantitation of the area of oligodendrocyte loss in PLPy/+ and PLPy/– animals injected with MS#30 plus HC (n = 6 PLPy/+ and 7 PLPy/ ICIs) and ON#34 plus HC (n = 4 PLPy/+ and 4 PLPy/– ICIs) (Mann-Whitney U test, *P < 0.05; **P < 0.01).